DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment

Answers

Answer 1

one low-thickness and one middle-of-the-road thickness band. An organic chemist disconnects and decontaminates particles required for DNA replication.

coli cells become on a 15N medium are moved to a 14N medium and permitted to develop for two additional ages (two rounds of DNA replication). DNA extricated from these cells is centrifuged.

The processed DNA (5 to 10 kb; 10 to 50 mg/ml) was utilized to change skillful E. coli cells, however, no transformants were recuperated. Roundabout plasmids are significantly more productive for change than straight structures are (4).

Escherichia coli filling in a supplemented medium containing glucose don't use the milk sugar, or lactose (glucose-4-β-d-galactoside); be that as it may, on the off chance that the microorganisms are set in a development medium containing lactose as the sole wellspring of carbon, they combine β-galactosidase and can in this manner use lactose.

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Related Questions

help please i need a sentence of this

Answers

Answer:

one threat is pullution

Explanation:

pollution will make the water dirrty and we can no longer use that water

What mutation did Lucy have?

Answers

Due to Lucy's high levels of deoxyadenosine in her blood, I was able to discover she had ADA-SCID. Her proteins were produced improperly and were shorter due to the stop codon mutation.

Since the gene mutation has no impact on the active site, there is no issue. Lucy's seizures were found to be caused by a mutation in the STXBP1 gene, which also causes the rare neurological disorder STXBP1 encephalopathy, following the completion of a genetic test known as an epilepsy panel.

I believe that the tRNA error is what is causing Lucy's ADA enzyme to malfunction. Lucy's 1500 base pair length is in line with the chart's usual range for gene length. However,  Lucy only has half of that at 283.

The complete question is:

Lucy was admitted to CHOP. Because Lucy did not have an infection or an injury to her brain that was causing her seizures. What mutation did Lucy have?

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How many waves are passing from y through n?
A.) about 10
B.) about 15
C.) about 20

Answers

Answer: b

Explanation:

1)Sam needs 10 kg of lemons to make lemonade.If each of the bag weighs 1000 g how many bags does he need?

2)How many 250 g bags of grapes are needed to make 5 kg?

Answers

Answer:

1) 10

2) 1000g

Explanation:

1000g =1kg

total bags needed = 10

5000g = 5kg

5000g /5

total 250 bags of grapes are needed = 1000g

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what are the agents of pollination?please answer​

Answers

Answer:

Explanation:

Pollinators range from physical agents, especially the wind (wind pollination is called anemophily), or biotic agents such as insects, birds, bats and other animals (pollination by insects is called entomophily, by birds ornithophily, by bats chiropterophily).

Answer:

hope it helps..stay safe

Explanation:

Pollinators range from physical agents, especially the wind (wind pollination is called anemophily), or biotic agents such as insects, birds, bats and other animals

need help asap
question 4
- 20 points

Answers

The second one I think, I’m not to sure

Does massage loosen tight muscles?

Answers

Yes, massage can lossen tight muscle.

In general ,Massage will help in  relaxation of tight muscles, by separating and loosening muscles fiber. Muscles that are elongated and stretched after message can releases tension which helps in easy movement.

Hence, the most common types of massage that should be advised for relieving tight muscles comprises of deep tissue massage  and remedial massage. Out of which Deep tissue massage are used for relieving tight muscle by treating the inner most or deeper layers of muscle and fascia. Some times Stretching and applying hot and cold pack in alternating manner can also helps in loosening of tight muscle.

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describe at the molecular level how cells extract energy frm starches proteins and lipids by the process of aerobic respiration

Answers

Starches, proteins, and lipids are broken down by enzymatic reactions into things such as glucose, amino acids, and fatty acids.

What is the genotypic ratio of 50%?

Answers

A 1:1 phenotypic ratio occurs when two possible genotypes have a 50% chance of developing when organisms are crossed. The chances of getting the ratio to 50% depicts both the recessive as well the dominant traits all together.

Phenotype, a person's or a species' observable traits and attributes, is influenced by genotype. Depending on the trait, genetics can impact phenotypic to varying degrees. For instance, genotype alone determines the colour of a pea plant's petals. Depending on the alleles present, the pea plant's petals can be either purple or white. But the genotype has a very small impact on some traits. These traits are occasionally referred to as complex traits since they are impacted by a variety of variables, including environmental and epigenetic influences. Due to the influence of environment and growing conditions, even individuals with the same genotype do not all look or behave the same.

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Tiene como objetivo eliminar microorganismos causantes de enfermedad

Answers

Answer:

Mantenha sempre as mãos limpas

Siga dicas para tossir e espirrar sem contaminar.

Evite tocar em seu nariz, olhos e boca com as mãos sujas. ...

Evite tocar em seu nariz, olhos e boca. ...

Evite o contato com pessoas que estão doentes, pois podem estar contidas.

What role does fossil fuels play in the carbon cycle? *
They are a sink of carbon. The carbon in fossil fuels hasn't been in the carbon cycle in
millions of years.
They absorb carbon dioxide from the atmosphere with their buffering ability
The fossil fuels are getting more acidic because they are absorbing too much carbon
dioxide
Fossil fuels are not involved in the carbon cycle. They are involved in nutrient cycling.

Answers

Answer:

A

Explanation:

Carbon moves from fossil fuels to the atmosphere when fuels are burned. When humans burn fossil fuels to power factories, power plants, cars and trucks, most of the carbon quickly enters the atmosphere as carbon dioxide gas. Each year, five and a half billion tons of carbon is released by burning fossil fuels.

20 POINTS,

IS water a non-renewable or renewable source?

Answers

Answer:

renewable sources.

Explanation:

Answer:

renewable

Explanation:

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Answers

Answer:

Chemical Weathering

Explanation:

It’s Mechanical weathering

You are on roller blades on top of a small hill. Your potential energy is equal to 1,000.0 joules. The last time
you checked your mass was 60.0 kilograms.
a. What is your weight in newtons?



c. If you start skating down this hill, your potential energy will be converted to kinetic energy. At the
bottom of the hill, your kinetic energy will be equal to your potential energy at the top. What will be
your speed at the bottom of the hill?

Answers

The weight is 600 NThe speed is 5.8 m/s

What is the energy?

We know that energy has to do with the ability to do work. Now we ought to know that the energy that is contained in an object that is just at a fixed position can be said to be potential energy while the energy that is in an object that is in motion is said to have the kinetic energy.

We know that both the kinetic energy and the potential energy or both the forms of mechanical energy and that the potential energy would be converted to kinetic energy as the object is falling down to the bottom.

a) The weight of the object is;

Weight = mass * acceleration due to gravity

Weight = 60 Kg * 10 m/s^2

= 600 N

c) Given that kinetic energy = potential energy

1000 = 1/2mv^2

v = √2 * 1000/60

v = 5.8 m/s

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Plantas de la herbolaria mexicana que usamos para calmar problemas digestivos??? AYUDAAA ES PA HOY ANTES DE LAS 10pm

Answers

Answer:

Epazote

Explanation:

Epazote is the Mexican herb that can be use to treat digestive problems. The health benefits of epazote include reduce flatulence, eliminate parasites that are present in the digestive system and in the body, improve metabolism, protect our immune system, causes weight loss, heal the wounds, and reduce menstrual discomfort. This herb is very effective in preventing digestive disorders.

What is the process of passing genes?

Answers

Transcription and translation are the two main procedures. Gene expression is the result of transcription and translation working together.

The information contained in a gene's DNA is transferred to an analogous molecule called RNA (ribonucleic acid) in the cell nucleus during transcription. However, when people discuss the passing on of genes, they typically do not refer to the passing on of genes during cell division from one cell to another.

Instead, they typically refer to genes that are passed on from parents to their offspring. Heredity or "inherited genes" refer to this. An egg from the mother and sperm from the father combine to make a fetus. Each chromosome in the egg and sperm makes up one half of the complete set. The embryo is created from the sperm and egg.

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Notice the two possible arrangements of chromo-somes in late prophase I. Considering what you know about DNA replication and meiosis, is either arrangement equally likely during the formation of tetrads in late prophase I

Answers

Yes, both arrangements of chromosomes in late prophase I are equally likely during the formation of tetrads. This is because, during meiosis, the homologous chromosomes replicate and then line up in pairs, known as tetrads.

During this process, the chromosomes can line up in any order, as long as the homologous chromosomes are paired together. Since the two arrangements of chromosomes in late prophase I consist of homologous chromosomes, they are both equally likely during the formation of tetrads.

Furthermore, during meiosis, the homologous chromosomes randomly separate and move to opposite sides of the cell in anaphase I, so the order of the chromosomes in late prophase I will not affect the outcome of meiosis.

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Every deliberate action you make, such as pedaling a bike, walking, scratching, or smelling a flower, involves neurons in the __________ nervous system.\

Answers

Every deliberate action you make, such as pedaling a bike, walking, scratching, or smelling a flower, involves neurons in the Somatic  nervous system..

So , The correct option is B

The somatic nervous system is one of the peripheral nervous system's divisions. Only the skeletal muscles get motor signals from the central nervous system via the somatic nervous system.

The reactions that are consciously controlled are generated by the skeletal muscles. As a result, the somatic nervous system governs the body's voluntary motions.

Voluntary motions of skeletal muscles, such as walking, sniffing a flower, cycling, and so on, are therefore governed by neurons of somatic division.

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Full Question ;

Every deliberate action you make, such as pedaling a bike, walking, scratching, or smelling a flower, involves neurons in the ________ nervous system.

a) sympathetic

b) somatic

c) parasympathetic

d) autonomic

Explain the global pattern of Earth's Biomes

Answers

Answer:

The global pattern of Earth's Biomes is a function of climatic conditions, such as latitude, air pressure, and winds

Explanation:

The global pattern of Earth's Biomes includes the following:

1. Polar region: it located in the north and south pole of the earth

2. Temperate deciduous forest: easily located in Europe and the United States. It grows well during a mild and wet climate.

3. Temperate grassland: this is common in Hungary, South Africa, Argentina, and the USA. It is characterized by average rainfall and a mild climate.

4. Desert: this is peculiar to areas such as Tropics of Cancer and Capricorn. It is characterized by hot climatic conditions

5. Tropical rainforest: it is common to places mear equator, and it is characterized by hot and wet climate

6. Savanna grassland: usually located in central Africa, southern India, northern Australia, and central South America. It is characterized by heat and dry climatic conditions.

7. Coral reefs: usually found around the equator approximately 30° north and south from the equator. It is highly distinct naturally.

deep in your digestive system, next to your small intestine, you have a small organ called an appendix. your appendix is a (mostly) useless organ and does not help your digestion.what kind of structure is it and how do you know

Answers

Answer: The appendix is a vestigial organ located at the lower right side of the abdomen. Its structure is like a thin tube of about 2-20cm. This can be known through its location at the lower end of the cecum which is a pouch-like structure in the large intestine.

Explanation: The appendix is located in the region of the body where the small intestine connects to the large intestine. It's a short tube that's roughly an inch and a half in diameter. In most people, the appendix is located in the right bottom quadrant of the abdomen.

It is not known what role the appendix plays in the body. There is a school of thought that suggests the appendix works as a repository for healthy bacteria, "rebooting" the digestive system after an illness that causes diarrhea. Some medical professionals are of the opinion that the appendix is nothing more than an evolutionary relic that serves no use. There are no obvious negative health effects associated with surgical removal of the appendix.

Why do we think of wind as another form of solar energy?

Answers

Answer:

wind is produced by the uneven heating of the earth's surface by the sun

Explanation:

therefore, the sun is technically making windmills spin to make energy

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Identify: What is the name for the division of the nervous system that handles involuntary actions and processes of the body?

*15 Points* Please don't answer just for the points, actually answer the question.

Answers

The autonomic nervous system regulates involuntary and unconscious actions like breathing, digestion, and internal organ function. The system consists of two complementary parts; the sympathetic andparasympathetic systems.

Which statement is true about the part of the electromagnetic spectrum that is divided into seven ranges of wavelengths?
A. It is one of two parts visible to the human eye.


B. It is one of three parts visible to the human eye.


C. It cannot be seen by the human eye unless it rains.


D. It is the only part visible to the human eye.

Answers

Answer:

d

Explanation:

the only part visable to the human eye is visable light

A zygote is the same as a gamete, they are both haploid cells *
True
False

Answers

Answer:

False

Explanation:

The zygote is endowed with genes from two parents, and thus it is diploid (carrying two sets of chromosomes). The joining of haploid gametes to produce a diploid zygote is a common feature in the sexual reproduction of all organisms except bacteria.

Gametes are haploid cells, and each cell carries only one copy of each chromosome. These reproductive cells are produced through a type of cell division called meiosis.

What are 3 ways a mutation can occur in the DNA?

Answers

The three ways a mutation can occur in the DNA are, (1). Insertion

(2). Deletion (3). Translocation.

1. Insertion: The DNA strand receives more DNA nucleotides.

2. Deletion: The DNA sequence is altered as a result of the deletion of DNA nucleotides.

3. Translocation: The exchange of chromosomal segments.

Point mutations, or defects introduced in a single spot on a DNA molecule, include substitutions, insertions, and deletions. Sometimes errors can be significantly larger. Errors that relocate entire regions of a DNA molecule are known as chromosomal mutations, also known as gross or gene-level mutations. When cells divide and make duplicates of themselves, changes to the DNA sequence are called gene or genetic mutations. The human body is programmed with instructions from the DNA.

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what is the similar between an estuary zone and intertidal zone

Answers

An estuary zone and an intertidal zone are both areas along the coast where freshwater and seawater mix. Both are affected by the tidal movements of the ocean and support a wide variety of plants and animals adapted to living in these environments. Both estuaries and intertidal zones are important ecosystems that provide habitat for many species and support economic activities such as fishing and tourism. They are also vulnerable to pollution and other human impacts and are often protected and preserved

If long-term higher global temperatures continue to rise on Earth which aspect of the water cycle will most likely occur more rapidly?

Answers

Answer:

evaporation

Explanation:

because the more heat that hits the water the more it evaporates

If long term higher global temperature continue to rise on Earth which aspect of the water cycle will most likely occur more rapidly

The correct answer is :

It affects the evaporation and precipitation.

What is water cycle?

It is the cycle process by which water circulates between the earth's oceans, atmosphere, and land. It involves precipitation as rain and snow, drainage in streams and rivers.This precipitate water returns to atmospheric by evaporation.

What affects the parts of water cycle?

Change in climate affects the parts of the water cycle to speed up if the temperature increases.Than it leads to increase in the rate of evaporation. Increase in evaporation cause more precipitation.

So, evaporation is the correct answer.

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Teo proteins are formed from the same amino acids but have different functions suggest two factors which make from these proteins different

Answers

Answer:

Different sequence of amino acid and sequence of DNA.

Explanation:

All proteins are made of same amino acids but differ in their function because of different sequence of amino acids. The presence of different sequence is dictated by the nucleotide sequence of their genes. This different sequence of amino acid and sequence of DNA are the factors that are responsible for the different function of different proteins so we can conclude that sequence of amino acid and sequence of DNA are responsible for difference in function of proteins.

Can a female calico get pregnant?

Answers

No, calico cats can't breed, so they can't have children.

The calico cat is one of the most beautiful creatures in the world. Characterized by its unique fur, it is loved not only by cat lovers, but also by non-cat lovers.

Calico cats cannot actually breed due to their unique genetic makeup (and the fact that male calico cats are born sterile). The XXY configuration can lead to Klinefelter's Syndrome, a health problem we'll discuss later. These cats are too sterile and cannot be used to lineage other calico cats. Calico cats are believed to bring good luck and wealth to homes and families. It's also considered a little magical because it's three colors.

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Which of the following is true about ECT today?
(1 Point)

A.Patients experience severe pain during the shocks.

B.It is applied to both hemispheres of the brain.

C.Doctors prefer to use it first, before trying drug therapy.

D.It brings no discomfort to the patient.

Answers

Answer: B. It is applied to both hemispheres of the brain.

Explanation:

ECT or electroconvulsive therapy is the treatment which is given for the treatment of depression, mental depression, suicidal patients, and others. In bilateral process one electrode is applied on the left and other on the right side of the brain. This way both hemispheres get the electrical treatment. Hippocampus and amygdala are two parts of the brain which are targeted in this process. These parts control the memory and emotion.

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