in gel electrophoresis, a loading dye is usually added to the sample of dna to be loaded in the wells of the gel. why would this be important?

Answers

Answer 1

Answer:

Gel loading dye is typically made at 6X concentration (0.25% bromphenol blue, 0.25% xylene cyanol, 30% glycerol). Loading dye helps to track how far your DNA sample has traveled, and also allows the sample to sink into the gel. ... It is important to use the same running buffer as the one used to prepare the gel.


Related Questions

which process is most critical for different types of tree cells to develop?

Answers

cell differentiation! :)

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Discussion 4.3: Preventing Heart Disease
Heart disease is generally caused by a mix of genetics and poor lifestyle factors such as high stress, poor diet, and lack of exercise. What can you do to prevent heart attacks, high blood pressure, and high cholesterol?

Answers

Answer:

Live a healthy lifestyle and avoid anything that may trigger such Diseases.

the ventral rami of spinal nerves ________ form the major nerve plexuses.

Answers

Answer:

The blank is:

C1 through T1 and L1 through S4

Hope this Helps

A frameshift mutation that results in the insertion of three nucleotides is often less deleterious than a mutation that results in the insertion of one nucleotide. Why?

Answers

A frame shift mutation that results in the insertion of 3 nucleotides doesn’t reading frame of the downstream codons and proteins they will be made into.

A mutation that inserts a single nucleotide ultimately will change the reading frame and all of the codons (3 nucleotides) downstream of that insertion will make a different protein than they were suppose to or a stop codon could be made which will completely stop that protein from being made.

Example: AUG UAU ACU CAC
The example above will encode for the following proteins.

met, Tyr, Thr, His

3 nucleotide insertion in this example could be:

AUG UAU AAU ACU CAC

met, Tyr, Asn, Thr, His

1 nucleotide insertion mutation:

Example: AUG UAA U ACU CAC

Met, stop codon

Nucleotides after the A insertion are not made into proteins.

place each of the labels in the proper position to denote whether they would pass through the filtration membrane or not.

Answers

The labels that will pass through the filtration membrane are water, glucose, and sodium.

The labels that will not pass through the filtration membrane are red blood cells, lymphocytes, antibodies, and platelets.

The filtration membrane is a microporous glomerular barrier membrane made of polymeric materials that are used for the separation and filtration of substances based on their molecular weight.

Depending on the molecular size of a substance situated in the blood, we can determine if such substances can be filtered through the filtration membrane or not.

However, the lower the molecular weight of the substance, the higher their likelihood of filtration.

In the plasma, the substance that has a lower molecular weight are water, glucose, and sodium while substance associated with protein, blood cell, and platelets contains higher molecular weight.

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the entire period between one m phase and the subsequent m phase is called what?

Answers

Explanation:

Interphase: The entire period between one M phase and the subsequent M phase is called: interphase.

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Blood must travel through capillaries at a(n) ________ rate to assist with diffusion of fluid into the interstitial space.

Answers

Blood must travel through capillaries at a slow rate to assist with diffusion of fluid into the interstitial space.

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In camellia plants, flower color is controlled by a single gene with codominant alleles. A camellia plants with red flowers (RR) is crossed with a camellia plant with white flowers (WW). What are the expected phenotypes of the offspring of this cross?

Answers

Answer:

All will have both red and white flowers because the incomplete dominance have two combined alleles given for the offspring with intermediate phenotypes. Then offspring will be pink coloured while in codominance all alleles are expressed in phenotype in the offspring together so the red and white flowers both will be shown.

Explanation:

Helpp

How might human population growth result in a tragedy of the commons?

A. Each birth is a common resource for the population.

B. All people use natural resources to survive.

C. People are the most important resource on Earth.

D. People have more in common than they realize.

Answers


As the human population grows, the Earth and its resources remain constant. As each of us focuses more naturally on personal betterment and gain, common resources become overloaded and dwindle – this is the Tragedy of the Commons.

Answer:

B. All people use natural resources to survive.

Explanation:

Just got it right on my quiz :)

Explain how hydrogen fuel cells work, and why they provide a healthy, clean way to produce heat and electricity. (Site 1).

Answers

Answer:

As there are no moving parts, fuel cells operate silently and with extremely high reliability. Due to their chemistry, fuel cells are very clean. Fuel cells that use pure hydrogen fuel are completely carbon-free, with their only byproducts being electricity, heat, and water.

Answer:

As there are no moving parts, fuel cells operate silently and with extremely high reliability. Due to their chemistry, fuel cells are very clean. Fuel cells that use pure hydrogen fuel are completely carbon-free, with their only byproducts being electricity, heat, and water.

8. Name three things that form during the cell cycle.​

Answers

Answer:

Hair, Blood Cells, and Skin

You observe a yellow zone surrounding growth on mannitol salt agar. What does this indicate about the organism

Answers

Answer:

The organism is able to ferment mannitol.

Explanation:

Find density if mass equal 400lb volume 20

Answers

Answer:

just use the formula d=m/v and plug in the numbers to solve the equation

Explanation:

enzymes that cleave nucleotides from the ends of a dna molecule are known as what?

Answers

Restriction enzymes. These enzymes are commonly found in bacteria and are able to cleave DNA at specific parts within DNA

Enzymes that cleave nucleotides from the ends of a DNA molecule are known as Restriction endonucleases.

What are Enzymes?

Enzymes may be defined as the type of biocatalysts that significantly increase the rate of a chemical reaction without being changed in the overall process. It is thought that all cellular reactions and processes are mediated by enzymes.

Restriction endonucleases are the enzymes that are also called molecular scissors which are responsible for cutting DNA at specific sites. These enzymes are present in bacteria in order to provide a defense mechanism called the restriction modification system.

There are various types of restriction enzymes are known. For example, BamH1, EcoRI, Hind III, etc.

Therefore, restriction endonucleases are the enzymes that cleave nucleotides from the ends of a DNA molecule.

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The ordered array of an organism's chromosomes is called a(n)


allele


pedigree


karyotype


mutation

Answers

Answer:

allele is the best answer there

Answer:

I understand you probaly don't need this answer anymore but the correct answer is Karyotype.

Explanation:

How do organisms remain highly organized in spite of the tendency toward entropy

Answers

Answer:Energy stored by organisms in organic molecules

Explanation:

the key character that reptiles have and amphibians do not have is a/an

Answers

Answer:

Reptiles have scales, and their skin is dry. Amphibians do not, and their skin is often moist with mucus, which keeps them from drying up.

What is the open space that separates the left and right lung?

Corpus callosum
Septum
*Mediastinum
All of the above

Answers

Answer:

c. Mediastinum

Explanation: is correct. post protected

identify the explanations researchers suggested to explain differences in health and mortality by gender.

Answers

The differences in health and mortality by gender is a phenomenon for which researchers have many possible explanations.

There have been studies that have indicated that there is enough variation in the average health of males and females to conclude that there are factors at play that are affecting health.

Amongst the many possibilities, scientists have mentioned:

Gender-reliant genetic vulnerability Hormonal factorsDifferences in physiology

as the main possible reasons.

Hormones play a big part in the health of each individual and are vastly different between genders, along with the endless differences in physiology at a molecular level. These factors together may explain why some members of a gender are more vulnerable to genetic illness.

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what type of carbohydrate is created by photosynthesis?

Answers

Answer:

glucose

Photosynthesis requires sunlight, carbon dioxide, and water as starting reactants (Figure 5.5). After the process is complete, photosynthesis releases oxygen and produces carbohydrate molecules, most commonly glucose.

2) What are the primary functions of all the Organic Compounds/Macromolecules?

Answers

Answer:

enter in formation of the body

Explanation:

provide cells with energystore energyhelp from cell membrance


Directions: Together with your parents and siblings, create a simple irrigation system in your house that will help in growing your plants. Watch the video on the link below and use the knowledge that you gained from the lesson. After doing the activity answer the question: "How did you apply the knowledge you gain from the video and from the lesson in creating your own irrigation system?

Knowledge and understanding
20
Students showed excellent knowledge about the topic
10
Students showed limited knowledge about the topic
5
Students have very limited knowledge about the topic​

Answers

The video with the necessary information to answer the question is missing. So, an irrigation system definition and example will be given.

An irrigation system is a structure made of tubes, hoses, and sprinkles; Its function is to water plants or crops with the correct amount of water and in an evenly way.

A simple irrigation system that will help grow plants at home is a plastic bottle with small holes connected to a hose with a tube that can lead the water from the hose to the bottle without losses. The bottle with its holes works as a sprinkler.

In conclusion, irrigation systems are in lawns, fields, or gardens, and they water the plants evenly so that they can grow. There are many types, but we can build a simple one with a bottle.

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Fermentation occurs in the
presence of ATP
absence of ATP
presence of oxygen
absence of oxygen

Answers

Answer:

Fermentation occurs in the absence of oxygen.

Mention some of plant organs​

Answers

Answer:

Root,stem,Leaf and flower

Answer:

Plants are made up of organs, including roots, leaves, the stem and reproductive organs.

Each organ has its own functions.

Together, the organs of a plant allow it to carry out the seven processes of life.

Explanation:

Red blood cells have _________ and generate energy ________.
mitochondria; anaerobically
no mitochondria; anaerobically
mitochondria; aerobically
no mitochondria; aerobically
None of the above

Answers

Answer:

no mitochondria; anaerobically

Explanation:

Red blood cells have no mitochondria and generate energy anaerobic. So, the answer would be B. You can easily eliminate A. and C. because they mention that blood cells have a mitochondria, but they don’t.

which is a wildlife species category that state resource agencies may use

Answers

Answer: Large mammals, Small mammals, Upland birds, Waterfowl and wetland birds, Birds of prey.

Wildlife species are defined as undomesticated animal, plant, or organism that grows in the wild and are not introduced to humans. The species that can be used by the state resource agencies are large mammals, small mammals, birds of prey, and waterfowl.

The animals that can be hunted legally must be:

The animal can be used as food and should be useful. The population of the animals should be high. The hunters should have a challenge while hunting them.

Also, some species labeled as endangered must be protected by law. These species are on the verge of getting extinct and are present in the least numbers to harvest.

Thus, the state agencies may use large mammals, small mammals, and waterfowl.

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Do all cells (brain cells, skin cells, liver cells, etc) express the genes or make the same proteins

Answers

Answer:

yes

Explanation:

have a good day or night

why is the digestive tract of the frog well vascularized

Answers

The digestive tract of the frog is well vascularized because it aids in the absorption of nutrients.

The digestive tract of a frog is a hollow long tube that extends from the mouth to the anus which helps in the ingestion and digestion of food.

The digestive tract of frog has the following characteristics:

alimentary canal of the frog is not long, that is, it is short. It has accessory organs of digestion such as liver, gall bladder and pancreas.

All the digested food substances are absorbed at the small intestine due to the presence of microvilli.

This microvilli is made up of a central core that is vascularized, that is, it contains artery, vein and lymphatic capillary.

Therefore, the vascularized microvilli of the small intestine of frog help in the absorption of nutrients.

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Explain why bacteria and archaea are prokaryotic cells and all other organisms are eukaryotic cells

Answers

Answer:

Archaea and bacterial cells lack organelles or other internal membrane-bound structures. Therefore, unlike eukaryotes, archaea and bacteria do not have a nucleus separating their genetic material from the rest of the cell.

Explanation:

During meiosis, the cells that are created are used during
a. regeneration of new tissue
b. regeneration of worn out cells
c. fertilization
d. cytokinesis
e. more than 1 answer is correct

Answers

Answer:

C

Explanation:

because fertilization is the fusion of male gamete and female gamete that arises from meiosis

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