in the gel electrophoresis lab, you determined the ________ of three different dna samples.

Answers

Answer 1

In the gel electrophoresis lab, you determined the genotype of three different DNA samples.

Gel electrophoresis is a process that allows you to separate fragments of DNA based on size. You place different size fragments of DNA into a gel, then apply current to the buffer surrounding the gel, the DNA will travel through the gel but move at different speeds based on the different fragment sizes. Larger fragments of DNA will move slower than smaller fragment sizes.

Let consider two different alleles:

the bigger allele -- Athe smaller allele -- a

The bigger allele happens to contain a restriction site that is allowed to be cleaved by a restriction enzyme while the smaller allele has a mutation in the restriction site that means that it will not be recognized and cleaved by that same restriction enzyme.

Let us remember that the person will have two copies of this gene and can be:

H0m0zygous dominant AAh0m0zygous recessive aaheterozygous Aa

An individual with h0m0zyg0us AA only have versions of the gene that will be cut by the restriction enzymes, each allele producing a small fragment of DNA and a medium-size fragment of DNA

An individual with the genotype aa only has the version of the gene that does not have the restriction site and therefore does not get cut by the restriction enzyme. The DNA sample from this individual will have a single larger fragment of DNA.

When you isolate DNA from heterozygous Aa, half of the DNA isolated has the allele with the restriction site that gets cut into two fragments while the other half will have the allele that does not have the restriction site and remains whole when a restriction enzyme is added.

Therefore, we can conclude that in the gel electrophoresis lab, you determined the genotype of three different DNA samples.

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Related Questions

NEED URGENT HELP!

A. What are three things that can create microclimates?

B. What characteristics of these things help create a microclimate?

Answers

Topography (height), large bodies of water and urban areas can create microclimates

in a hot place, if theres a mountain there, the top of the mountain can be cold

cement in an urban area can absorb a lot of heat during the day and then release it at night

valleys can have cold air

Can somebody please help me on this I just got this brainly app and I really want to give more points if you help me But this is as much as I have (BTW THIS IS SCIENCE)

Answers

Answer:

the answer would be (c)

Explanation:

hope this helps

True/False: In a neuron at rest, the concentration of sodium ions is 14 times higher outside the cell than inside the cell.

Answers

Answer:

true

Explanation:

The sodium and chloride ion concentrations are lower inside the cell than outside, and the potassium concentration is greater inside the cell. These concentration differences for sodium and potassium are due to the action of a membrane active transport system which pumps sodium out of the cell and potassium into it.

Aupporting cells of the nervous system are collectively called:

Answers

Supporting cells of the nervous system are collectively called neuroglia.

Answer:

They are called neuroglia or glial cells.

What diagnostic test is useful for detecting hyperactivity of the thyroid gland during an evaluation of a thyroid disorder

Answers

Answer:

Blood tests that measure thyroxine and thyroid-stimulating hormone (TSH) can confirm the diagnosis. High levels of thyroxine and low or nonexistent amounts of TSH indicate an overactive thyroid. The amount of TSH is important because it's the hormone that signals your thyroid gland to produce more thyroxine

Explanation:

The diagram above shows the cell cycle. As you can see from the diagram, the cell spends most of the time in _______________________ during which it undergoes rapid growth and the DNA is replicated in order to prepare for Mitosis

Answers

Answer:

Interphase

Explanation:

Interphase is the phase in the cell cycle in which rapid growth occurs and DNA is replicated.

Explain why diseases result in symtoms

Answers

Infection happens when germs enter body leading to damaged cells

Which layer of the atmosphere is experiencing this storm?

Answers

Answer:

Troposphere

Explanation:

Almost all weather occurs in the lowest layer of the atmosphere, called the Troposphere. The exception being large storms like hurricanes and large Thunderstorms. These storms have clouds with great vertical development and the tops of these storms go into the Stratosphere.

What is metastasis and what body systems are involved in the process?

Answers

Answer:

Metastases most commonly develop when cancer cells break away from the main tumor and enter the bloodstream or lymphatic system. These systems carry fluids around the body.

Explanation:

In metastasis, cancer cells break away from where they first formed, travel through the blood or lymph system, and form new tumors in other parts of the body.

How can scientists use half-life to help date new rocks and fossils that are found?

Answers

Answer:

radioactive isotopes of elements decay

Explanation:

As radioactive isotopes of elements decay, they lose their radioactivity and become a brand new element known as a daughter isotope. ... The half-lives of several radioactive isotopes are known and are used often to figure out the age of newly found fossils.

What type of solution causes a cell to shrink in size?
O Isotonic
O Homeotonic
O Hypertonic
O Hypotonic

Answers

Hypertonic C. I think answer correct.

Design a solution to the Zebra Mussel invasion. What steps may be taken to reduce the negative impact of Zebra Mussels in the rivers and lakes in North America? Your response should include a logical way to reduce the number of Zebra Mussels. You should include 2-3 steps regarding how your method will work.

Answers

Answer: See explanation

Explanation: Zebra mussels are creatures that latch on to boats and inhabit areas that they are supposed to be in. To reduce the influx of Zebra mussels you can

1. Always check the bottom of your boat before coming back from a different area.

2. If you see zebra mussels you can use high pressure hot water to get them off.

3. Mark the area zebra mussels are located so you can warn others to look under their boats when they are coming back from that area.

Hope this helps!

According to the dihybrid cross below, what proportion of peas would
be green and smooth?
A) 9/16
B) 1/4
C) 3/8
D) 1/16

Answers

Answer: is A. 9/16

Explanation:

Give an example of display behavior exhibited by a territorial animal.

Answers

Answer:

Agonistic (aggressive) displays usually occur near the borders of a territory. When a strange howler monkey approaches the territory of others, resident males set up a tremendous din, warning the intruder off. Many songbirds sit on highly visible perches while singing, providing both auditory and visual displays.

Is scientific law a proven concept? Explain why or why not.​

Answers

n general, a scientific law is the description of an observed phenomenon. It doesn't explain why the phenomenon exists or what causes it. The explanation of a phenomenon is called a scientific theory. It is a misconception that theories turn into laws with enough research.

One function of the nervous system is to maintain ____________________, or stability within the body

Answers

Answer:

Answer: Maintain body homeostasis with electrical signals.

Explanation:

Your nervous system is your body's command center. Originating from your brain, it controls your movements, thoughts and automatic responses to the world around you. It also controls other body systems and processes, such as digestion, breathing.

compared to parasympathetic ganglia, sympathetic ganglia are __________.

Answers

Compared to parasympathetic ganglia, sympathetic ganglia are autonomic ganglia of the sympathetic nervous system that prepare the body for emergency situations, such as: defense or flight reactions to a dangerous situation.

In the parasympathetic ganglia, the axons of neurons are characterized by being very long and reach the very structure of the target organ before making contact with the second neuron.

On the other hand, these ganglia activate the parasympathetic system that has to do with energy recovery situations such as: activating digestive secretions, reducing blood flow to the brain, which causes drowsiness.

In the sympathetic ganglia, axons originate from neurons located in the lateral horns of the spinal cord and exit through the anterior roots of the spinal nerves, along with those of the motor neurons.

These ganglia activate the sympathetic system that prepares the body for dangerous situations causing: increased heart rate, blood pressure, respiratory rate, etc.

Therefore, we can conclude that compared to parasympathetic ganglia, sympathetic ganglia are autonomic ganglia of the sympathetic nervous system that prepare the body for emergency situations.

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When Emma first began dancing, her movements were ______ and awkward. Now, after several months of practice, Emma is able to produce fluid, smooth movements as a result of which of the following?

*all of the above is correct

Answers

Answer:

All of the above is the correct answer

Explanation:

14.

a) Find m

B
C
:
degrees

b) Find m∠CED:
degrees

c) Find m

C
E
D
:
degrees

d) Find m

C
D
E
:
degrees

Answers

I also have the same problem

SOMEONE PLEASE HELP

When does renal genesis usually develop?

*it's b. despite the test having a typo

Answers

Answer:

b. In the womb, when the fetus is about 5-12 weeks old

Explanation:is correct. post protected

PLEASE HELP ANY ANSWER WILL DO PLEASEEEE PLEASE
Draw a tree diagram for the dichotomous key that you used for the aliens.

Answers

Answer:

Explanation:

HELP PLEASE SHOW WORK

1. How many grams are there in 2.3 moles of C?


2. How many moles are there in 43 grams of aluminum?


3. How many atoms are there in 100.5 moles of U?


4. How many moles are there in 6.818 x 10 26atoms of Ne?

Answers

Answer:

1. 46grams

2. 5moles

3. 476 atoms

4. 6,995,268

A key limitation of the coefficient of community is that it lacks the ability to:

Answers

Answer:

calculate the similarity between two communities.

Explanation:

Part 3

What type of front is front 1? What types of weather conditions take place there?

U

Font Sixes

Characters used 0 / 15000

Submit

Answers

Answer:

Front 1 is a cold front.

Explanation:

At a cold front, cold air moves in, causing a drop in temperature.

Help meeeeeeeeeeeeee

Answers

Answer:

1)sunlight and co2

2)oxygen and ?

Explanation:

A population of 200 mice contains 168 brown mice. Brown is dominant to gray. How much of the population would be h e t e r o z y g o u s?
A. 32%
B. 84%
C. 48%
D. 16%

Answers

The answer is B, yo welcome

The heterozygous population in the population of 200 mice is 84%. The correct option is B.

What is heterozygous?

Heterozygous means having two types of alleles for the same type of gene. These alleles show two types of characters for a single gene. An example is a person having an allele of brown and black hair.

The population is 200 of which 168 mice are brown

By the Hardy–Weinberg (HW) equilibrium

q² (bb) = (200 - 168) /200 = 32 / 200 = 0.16

q: √ 0.16 = 0.4

p = 1 - q = 1 - 0.4 =  0.6

p² (BB) = 0.6 x 0.6 = 0.36

Thus, the correct option is B. 84% regarding the heterozygous population.

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Based on cell theory, which of these answer choices would describe one reason a virus is considered nonliving?

Answers

Answer:D

Explanation:

I just did it

Scientists often use fruit flies as a method to test hypotheses about human genes. Why are fruit flies advantageous in the study of human inheritance?
They can self-pollinate.
They lack nucleic acids.
They are very different from humans.
They reproduce quickly and take up little space.

Answers

Fruitflies are advantageous in the study of human genes and inheritance because they reproduce quickly and take up little space.

Advantages of using Fruitflies in the study of human genetics

Fruitflies are usually a subject for the study of human genes and inheritance for some reasons:

They have a simple genetic structureThey reproduce quicklyThey are quite small and hence take up very little space

The quick rate of reproduction makes fruitflies good subjects for studying mutation as well.

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They reproduce quickly and take up little space.

What is inheritance?

Fruit flies (Drosophila melanogaster) are advantageous in the study of human inheritance because they have several characteristics that make them well-suited for genetic research. One key advantage is their rapid reproductive cycle, with a generation time of about two weeks. This allows researchers to observe multiple generations in a short period, accelerating the study of inheritance patterns and genetic traits.

Additionally, fruit flies have a relatively small size, making it feasible to maintain large populations in a laboratory setting without requiring excessive space or resources. This allows for efficient experimental design and the ability to conduct large-scale genetic studies.

While fruit flies share some genetic similarities with humans, they are not identical to humans. However, their genetic and developmental processes have enough similarities to provide valuable insights into fundamental principles of inheritance and gene function, which can inform our understanding of human genetics.

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Define negative feedback in biology

Answers

Answer:

A negative feedback loop occurs in biology when the product of a reaction leads to a decrease in that reaction. In this way, a negative feedback loop brings a system closer to a target of stability or homeostasis

Explanation:

Negative feedback occurs when some function of the output of a system, process, or mechanism is fed back in a manner that tends to reduce the fluctuations in the output, whether caused by changes in the input or by other disturbances

Specialized cells that communicate with each other through electrical and chemical signals are called:

Answers

Answer:

neurons

Explanation:

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