Solving the Sequence of an Oligopeptide From Sequence Analysis Data Analysis of the blood of a catatonic football fan revealed large concentrations of a psychotoxic octapeptide. Amino acid analysis of this octapeptide gave the following results: 2 Ala 1 Arg 1 Asp 1 Met 2 Tyr 1 Val 1 NH4 1 The following facts were observed: a. Partial acid hydrolysis of the octapeptide yielded a dipeptide of the structure C O C N H H COOH H3C CH3 CH3 C CH H3 N b. Chymotrypsin treatment of the octapeptide yielded two tetrapeptides, each containing an alanine residue. c. Trypsin treatment of one of the tetrapeptides yielded two dipeptides. d. Cyanogen bromide treatment of another sample of the same tetrapeptide yielded a tripeptide and free Tyr. e. N-terminal analysis of the other tetrapeptide gave Asn. What is the amino acid sequence of this octapeptide

Answers

Answer 1

Answer:

The amino sequence of an octapeptide can be determined by :

i.Amino acids present in the peptide

ii.By the behaviour shown by the treatment of these material with different biological and chemical reactions

As shown ammonium was released from the peptide,which shows that the peptide contains either Asn or Gln.

As there is presence of Asp in the amino acid analysis which indicates that the octapeptide contains Asp.

Acid hydrolysis hydrolyzes the peptide bonds which yields a Ala-Val  which is the result of partial acid hydrolysis.

Chymotrypsin is on the C-terminal side of the aromatic amino acids Phe, Tyr, and Trp. The peptide has Tyr which indicates that  the two present tetrapeptides after chymotrypsin treatment are fourth and eighth amino acids i-e N--Tyr-C and Tyr as the fourth amino acid and Ala-Val somewhere in the octapeptide

As Arg is only present on the C terminal side  of the Trypsin cleavage therefore Arg must be the second or the sixth amino acid for the two dipeptides to form as a result of arginine treatment of a tetrapeptide

Cyanogen bromide cleaves on the C-terminal side of Met residues. For free Tyr to be produced from cyanogen bromide treatment of one of the tetrapeptides, Met must be either the third or seventh amino acid. Because Ala was present in both tetrapeptides, Ala must be either the first or fifth amino acid, present in the same tetrapeptide as Arg and Met,i-e N.-Tyr-C and Ala-Val somewhere in the octapeptide and Ala-Arg-Met as the first, second, and third or fifth, sixth, and seventh amino acids and Tyr as the fourth amino acid.

One of the tetrapeptides contains Ala-Val as its second and third amino acids and Asn as its N-terminal amino acid. Therefore two combinations are possible as below

N-Asn-Ala-Val-Tyr-Ala-Arg-Met-Tyr-C or N-Ala-Arg-Met-Tyr-Asn-Ala-Val-Tyr-C

Answer 2

Answer:

The amino aciid sequence of an octapeptide can be determined from the amino acids that are present in the peptide and from the results of different chemical and biological treatments.

One equivalent of ammonium was released fro the peptide, indicating that the peptide contains either Asn or Gln. The presence os Asp in the amino analysis indicates that the octapeptide contains Asn.

A) Acid hydrolysis hydrolyzes peptide bonds. The dipeptide Ala-Val is obtained by partial acid hydrolysis. The indicates that Ala-Val is present in the peptide

B) Chymotrypsin cleaves on the C-terminal side of the aromatic amino acids. Phe, Tyr, and Trp. The peptide contains only Tyr. The presence of tetrapeptides after the treatment of ohymotrypsin indicates that Tyr is the fourth and eighth amino acids.

N-Tyr-C and Tyr present as the fourth amino acid. in constrast, Ala-Val is present in the octapeptide

C) Trpsin cleaves on the C-terminal side of Arg and Lys residues. only Arg is present in the octapeptide. Arg must be the second or sixth amino acid, for two dipeptide to be formed from arginine during the treatment of one tetrapeptide.

D) Cyanogen bromide cleaves on the C-terminal side of Met residues. For free Tyr to be produced from cynogen bromide treatment of one of the tetrapeptides, Met must be either the third or seventh amino acid. Because Ala is present in both tetrapeptides, Ala must be either the first or fifth amino acid, present in the same tetrapeptide as Arg and Met.

In addition, presence of N-...-Tyr-C and Ala-Val in the octapeptide and Ala-Arg-Met as the first, second, third or fifth, sixth or seventh amino acids and Tyr as the fourth amino acid.

E) One of the tetrapides contains Asn as its N-terminal amino acid, and Ala-Val as its second and third amino acids. Two octapeptides are possible from this peptide analysis.

The amino acid sequence of the octapeptide is the following:

N-Asn-Ala-Val-Tyr-Ala-Arg-Met-Tyr-C:

N-Ala-Arg-Met-Tyr-Asn-Ala-Val-Tyr-C:

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Related Questions

Which of the following planets has ammonia clouds in its atmosphere?

Jupiter
Mars
Mercury
Venus

Answers

Answer:

Jupiter and Saturn has ammonia clouds in its atmosphere since the molecules of ammonia gas that are present in their atmospheres condense to form clouds. However, these ammonia clouds are more visible in Jupiter than in Saturn because of their lower altitude in Saturn.

Answer:

A

Explanation:

i took the exam

What are the steps to
separate iron fillings, water,
leaves?

Answers

Answer:

Wrap a magnet in plastic lunch wrap and move it through the mixture of the three solids. The iron filings will stick to the magnet. The filings can be removed by unwrapping the plastic from the magnet carefully! Mix the remaining salt and sand in water and stir.

Explanation:

What do we mean when we say a substance is "pure"?

Answers

Answer:

A pure substance is a substance that is only made up of atoms of one element and so it has fixed melting and boiling points.

Help me please I need help

Answers

Answer:

uijj uhh the yuk high uijj john

Which of the following is an example of a chemical name using prefixes?
O A. Carbon dioxide
O B. Magnesium sulfate
O C. Iron(ll) carbonate
O D. Potassium chloride

Answers

A.Carbon dioxide because I had the question on my test and I got it right

Answer:

A

Explanation:

help me please i don’t understand the problem

Answers

Answer:

Atoms are neither created or destroyed.

Explanation:

The end result of a chemical change does not create or destroy any atoms. Matter cannot be created or destroyed, meaning the same amount of atoms exist before and after the change.

A. Atoms are neither created nor destroyed

what are they waiting? or what are their waiting?

please correct me if I'm wrong​

Answers

Hm I’m confused, if you mean what are they weighting, it’s they :)

Which type of atom will attract electrons

Answers

Answer:

nuetrons and protons

Explanation:

Explanation:

the electron pair will be attracted more strongly to the atom that has the higher electronegativity

hope it help

(PLEASE HURRY) Which of the following statements is an example of quantitative data? A. There are ten butterflies. B. The butterflies are red with yellow spots. C. The butterflies are eating nectar from the flower. D. The butterflies are flying.

Answers

answer: a
explaination: it said it needs to be 20 characters but the answer is a

The diagram shows structures that represent different levels of organization in a human. Which is the simplest level of organization?
W
X
Y
Z

Answers

Answer: the answer is Z

Explanation:

Becuase the order from smallest to largest would be:

Y: Cell

Z: Tissue

W: Organ

X: Organ system

Is a nickel coin magnetic

Answers

Answer:

Yes

Explanation:

Because it was false because it has been the substance abuse permalink

yes they are magnetic

How many electrons are in the 4p orbital does Br possesses?

Answers

Answer:

5 electron

It contains 6 electrons in 2p orbital, 6 electrons in 3p orbital and 5 electron in 4p orbital.

The two alternative chair conformations of cis-1-bromo-2-methylcyclohexane differ in their Gibbs free energy. Using the data for ΔG (Axial-Equatorial) for monosubstituted cyclohexanes at room temperature (25ºC): Axial → Equatorial Group ΔG° (kJ/mol) Group ΔG° (kJ/mol) -0.8 -5.9 -2.4 -7.3 -3.9 1,2-gauche 3.8 Calculate the absolute value of the difference in the Gibbs free energy between the alternative chair conformations. kJ/mol Which group in this compound is in axial position in the energetically preferred chair conformation? _______

Answers

Answer:

a.) 4.9kj/mol

b.) -Br group

Explanation:

first all of your question did not make mention of the groups in the table.

here it is:-

axial group     ΔG°(kj/mol)    group     ΔG°kj/mol

CN                   -O.8                NH₂          -5.9

Br                     -2.4                 CH₃           -7.3

OH                    -3.9            1,2-gauche      3.8

The 6 axial groups are bonded one on each carbon and also they are parallel and also alternate from up to bottom.

a.)

to get the absolute ΔG;

ΔG = -2.4-(-7.3)

= -2.4+7.3

= 4.9KJ/MOL

b.)

the axial group in this compound which is in the axial position in the energetically preferred chain conformation is the -Br group

Please check attachment for diagram

2 What do you notice about the location of the elements in each family?​

Answers

Explanation:

They are indeed placed from the far left hand side of the periodic table in almost the same column. If you step around the table between left to right, the movement of electrons rises by one. When you progress down the line, the number of power increases by the one.

How many grams of chloride are there in 256 g of magnesium chloride?

Answers

Given :

Mass of given magnesium chloride, m = 256 g.

To Find :

How many grams of chloride are there in 256 g of magnesium chloride.

Solution :

Molecular formula of magnesium chloride is [tex]MgCl_2[/tex].

Molecular formula of [tex]MgCl_2[/tex] is, M = 95.211 g/mol .

Mass of chlorine in 1 mol of [tex]MgCl_2[/tex]  is , m = 35.5 × 2 = 71 g.

So, amount of chlorine in 256 gram [tex]MgCl_2[/tex] :

[tex]m =\dfrac{71\times 256}{95.211}\\\\m = 190.90\ g[/tex]

Hence, this is the required solution.

What is one way the lack of gravity in space affects the human body?
Please help ASAP

Answers

Answer:

Loss of muscle mass because there is no weight on the back and legs.

Explanation:

Which substance cannot be broken down by chemical changes? Magnesium Hydroxide Carbon Dioxide b O Ammonia Iron​

Answers

Answer:

Iron

Explanation:

The only substance that cannot be broken down by a chemical change is Iron.

Iron is an element and elements are made up of atoms. Atoms are the smallest indivisible particles that takes part in chemical reactions.

Other choices are compounds. Compounds are made by combining atoms together through the loss, gain or sharing of electrons. This interaction creates bonds between two or more species. But for substances made up of atoms alone, no bonds are formed and hence, they cannot be further broken down.

what is it called when an object is being partially or tottally blocked by another object in space​

Answers

An eclipse is when two celestial bodies lines up in space and one of the objects gets partially or totally blocked. This could happen when the sun blocks the moon, or when the moon blocks the sun. These are just some of the examples there are many others out there, so the answer would be Eclipse!


Hope this helps and stay safe!

Please help if you can !

Answers

Answer:

7630

Explanation:

eAnswer:

Explanation:

Math

Chocolate drink can easily be dissolved in

Answers

Answer:

Chocolate drink can easily be dissolved in Hot water than cold water.

Explanation:

Chocolate drink can easily be dissolved in Hot water than cold water because hot water has more heat or thermal energy than cold water. When water is heated and becomes hot, the molecule of water gain alot of energy and this make it to move very fast and as these molecules move faster, they begin to come in contact with the molecules of chocolate drink, thereby making it to dissolve faster than that of cold water.

Aspirin synthesis involves the addition of an acetyl group to salicylic acid in a condensation reaction with an alcohol. The acetyl group could be added with a carboxylic acid but the preferred procedure is to use the acid anhydride. Why is preferable to use an acid anhydride for ester formation with an alcohol rather than a carboxylic acid? Select one: Carboxylic acids react with water in an undesired side reaction. Acid anhydrides are generally easier and safer to handle than carboxylic acids. Carboxylic acids change the pH of the solution too much for the reaction to proceed. Acid anhydrides are less stable than esters so the equilibrium favors the ester product.

Answers

Answer:

The correct appropriate will be Option 1 (Acid anhydrides are less stable than esters so the equilibrium favors the ester product.)

Explanation:

Acid anhydride, instead of just a carboxyl group, is typically favored for esterification. The predominant theory would be that Anhydride acid is somewhat more volatile than acid. This is favored equilibrium changes more toward the right of the whole ester structure. Extremely responsive than carboxylic acid become acid anhydride as well as acyl chloride. Thus, for esterification, individuals were most favored.

The other options offered are not relevant to something like the scenario presented. So, the solution here is just the right one.

The reversible reaction, 2NO{g) ⇌ N2 (g) + O2 (g), would be classified as a __________________ reaction.
homogeneous
heterogeneous
endothermic

Answers

Endothermic!!! Energy be broken

HELP!!!!
An unknown gas effuses at a rate of 2.0 times the rate of CL2. What is the molar mass of the unknown gas?

Answers

The molar mass of the unknown gas is 17.75 g/mol  

From Graham's law of diffusion, we understood that the rate of diffusion of a gas is inversely proportional to the square root of the molar mass of the gas as shown below:

R ∝ [tex]\frac{1}{\sqrt{M}}[/tex]

Expanding further, we have

[tex]\frac{R_{1} }{R_{2} } = \sqrt{\frac{M_{2}}{M_{1}} }[/tex]

Where

R₁ and R₂ are the rate of diffusion of the individual gas

M₁ and M₂ are the molar masses of the individual gas.

With the above information in mind, we can obtain the molar mass of the unknown gas as follow:

Let R₁ be the rate of the unknown gas

Let R₂ be rate of Cl₂ gas

Let M₁ be the molar mass of the unknown gas

Let M₂ be the molar mass of the Cl₂ gas

From the question given above, we were obtained the following:

Rate of the unknown gas = 2 times the rate of Cl₂ i.e

R₁ = 2R₂

Molar mass of Cl₂ (M₂) = 2 × 35.5 = 71 g/mol

Molar mass of the unknown gas (M₁) =?

[tex]\frac{R_{1} }{R_{2} } = \sqrt{\frac{M_{2}}{M_{1}} }\\\\\\\frac{2R_{2}}{R_{2} } = \sqrt{\frac{7 1}{M_{1}} }\\\\2 = \sqrt{\frac{7 1}{M_{1}} }[/tex]

Square both sides

[tex]2^{2} = \frac{71}{M_{1}} \\\\4 = \frac{71}{M_{1}}[/tex]

Cross multiply

4 × M₁ = 71

Divide both side by 4

[tex]M_{1} = \frac{71}{4}[/tex]

M₁ = 17.75 g/mol

Therefore, the molar mass of the unknown gas is 17.75 g/mol

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how many atoms are in 20 grams of aluminum ​

Answers

Answer:

4.5 × 10²³ number of atoms Al

Explanation:

Given data:

Mass of Al = 20 g

Number of atoms of Al = ?

Solution:

The given problem will solve by using Avogadro number.

'It is the number of atoms , ions and molecules in one gram atom of element, one gram molecules of compound and one gram ions of a substance" .The number 6.022 × 10²³ is called Avogadro number.

We will calculate  the number of moles of Al first:

Number of moles = mass/molar mass

Number of moles = 20 g/ 27 g/mol

Number of moles = 0.74 mol

1 mole = 6.022 × 10²³ number of atoms

0.74 mol ×  6.022 × 10²³ number of atoms / 1 mol

4.5 × 10²³ number of atoms Al

The required number of atoms is [tex]4.46\times 10^{23}[/tex]

Atomic Weight:

Atomic weight, also called relative atomic mass, ratio of the average mass of a chemical element’s atoms to some standard.

Given that,

Mass=[tex]20[/tex] grams

1 mole of aluminum [tex]= 27g = 6.023\times10^{23}[/tex] number of atoms 27g of aluminum contains [tex]6.023\times10^{23}[/tex] a number of atoms.

Therefore,20 grams the number of atoms is,

[tex]6.023\times10^{23}\times\frac{20}{27} =4.46\times10^{23}[/tex]

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Which of the following is an example of physical change?

Answers

Answer:

The glass cup falling from the counter

Explanation:

the glass isn't changing in any chemical way. it's still made of the same material, just broken apart.

if i change it by looking at it what type of change is it ?
non impact
kinetic
physical
chemical

Answers

Answer:

the answer to this is non impact

Which list of elements from Period 2 is arranged in decreasing
ionization potential?

Answers

Answer:

It’s ionisation

Explanation:

In the reaction Zn H 2SO 4→ ZNSO4 + H2, which, if any element is oxidized

Answers

Answer:

I hope this answer is correct

Explanation:

This is an oxidation-reduction (redox) reaction:

Zn0 - 2 e- → ZnII (oxidation)

2 HI + 2 e- → 2 H0 (reduction)

Zn is a reducing agent, H2SO4 is an oxidizing agent.

Reactants:

Zn

Names: Zinc source: wikidata, accessed: 2019-09-07, Zinc powder (pyrophoric) source: ICSC, accessed: 2019-09-04, Zn source: wikidata, accessed: 2019-09-07

Appearance: Grey-to-blue powder source: ICSC, accessed: 2019-09-04

H2SO4 – Sulfuric acid source: wikipedia, accessed: 2019-09-27source: wikidata, accessed: 2019-09-07source: NIOSH NPG, accessed: 2019-09-02

Other names: Oil of vitriol source: wikipedia, accessed: 2019-09-27source: wikidata, accessed: 2019-09-07source: ICSC, accessed: 2019-09-04source: NIOSH NPG, accessed: 2019-09-02, Sulfuric acid, concentrated (> 51% and < 100%) source: ICSC, accessed: 2019-09-04, H2SO4 source: wikidata, accessed: 2019-09-07

Appearance: Clear, colorless liquid source: wikipedia, accessed: 2019-09-27; Odourless colourless oily hygroscopic liquid source: ICSC, accessed: 2019-09-04; Colorless to dark-brown, oily, odorless liquid. [Note: Pure compound is a solid below 51°F. Often used in an aqueous solution.] source: NIOSH NPG, accessed: 2019-09-02

Products:

ZnSO4 – Zinc sulfate source: wikipedia, accessed: 2019-09-27source: wikidata, accessed: 2019-09-02source: ICSC, accessed: 2019-09-04

Other names: White vitriol source: wikipedia, accessed: 2019-09-27, Goslarite source: wikipedia, accessed: 2019-09-27, Zinc sulfate (1:1) source: wikidata, accessed: 2019-09-02

Appearance: White powder source: wikipedia, accessed: 2019-09-27; Colourless hygroscopic crystals source: ICSC, accessed: 2019-09-04

H2

Names: Dihydrogen source: wikidata, accessed: 2019-09-07, Hydrogen source: ICSC, accessed: 2019-09-04source: wikidata, accessed: 2019-09-07, H2 source: wikidata, accessed: 2019-09-07

Which one of the following statements about the β-sheet is FALSE? The β-sheet is a type of regular secondary structure. β-sheets can be parallel or antiparallel. The β-sheet contains hydrogen bonds between the carbonyl oxygen of one residue and an amide hydrogen of a residue on an adjacent strand. The side chains in a β-sheet alternate between the two sides of the sheet. The β-sheet is a type of secondary structure that fulfills the hydrogen bonding requirements of amino acid side chains. g

Answers

Answer:

The correct answer is "The β-sheet is a type of secondary structure that fulfills the hydrogen bonding requirements of amino acid side chains".

Explanation:

Hydrogen bonding are among the most common chemical bonds that occur between amino acids, where one atom of an amino acid acts as an hydrogen donor and the other acts as hydrogen acceptor. It is not true that β-sheet fulfills the hydrogen bonding requirements of amino acid side chains. β-strands are directional and form hydrogen bonds in antiparallel, parallel, or mixed arrangements. In the parallel form adjacent amino acids do not form hydrogen bonds, but they bond with the residues that flank the other.

The statement related to β-sheet that is considered to be false is that it should be the type of the secondary structure.

What is Hydrogen bonding?

It is the bonding that should be arise mainly with the chemical bonds that lies between the amino acids where one atom should be acted as the donor of hydrogen and the other should be like an acceptor of hydrogen. Also, β-strands should be considered as directional and create the hydrogen bonds in the anti-parallel or mixed arrangements.

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Enter the cycle number, n, in the x column and the number of radioactive atoms in the y column. When you finish, resize the window.

Answers

Answer:

at first click done,

A: exponential

then, i just put 0.5, and somewhat close.

Explanation:

edge 2021!!!

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